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- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
(2E)-3-(2,3-Difluorophenyl)-2-propenoic Acid
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
236746-13-7
- 规格:
1g
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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
phosphate‐buffered saline (DPBS; with CaCl 2 and MgCl 2 ; e.g., Invitrogen) Cell lysis buffer: 50 mM Tris·Cl, pH 7.4 ( appendix 2E )/150 mM NaCl/1% (v/v) Triton X‐100/1× protease inhibitors
filter to remove oils from the gas) Argon Emery sheet (3/0 grit or finer) Ultrasonic processor equipped with a double‐stepped microtip (e.g., VCX750, Sonics & Materials) in a sound‐abating enclosure
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
, A., Bergqvist, S.P., Mandell, J.G., and Komives, E.A. 2004. Two different proteins that compete for binding to thrombin have opposite kinetic and thermodynamic profiles. Protein Sci. 13:166‐176.
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