- ￥970
- 源叶
- 中国
- V38589-1mg
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
2415761-65-6
- 规格：
1mg
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文献和实验Preparation of Segmented and Polarity Marked Microtubules
are microtubules with a bright seed and a dim elongated segment only on one end -- the plus end. Selective elongation of one end is achieved by inclusion of NEM-treated tubulin, a competitive inhibitor of minus end polymerization. More complex microtubule
Preparation of Segmented and Polarity Marked Microtubules
inhibitor of minus end polymerization. More complex microtubule substrates such as GDP microtubule lattices capped with segments of GMPCPP tubulin can also be prepared by playing around with in vitro polymerization conditions. Also, segmented and polarity
is RT; for tissue culture cells, tubulin willpolymerize better at higher temperatures. 30 - 33 deg.C is a good compromiserange to balance polymerization and proteolysis. Extracts must be supplementedwith a peptide protease inhibitor cocktail
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