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- Chemicon
- 654160-5MG
- 2025年10月01日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Tubulin Polymerization Inhibitor
- 规格：
5MG
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文献和实验Tubulin Polymerization with GTP/GMPCPP/Taxol
GMPCPP and add a small volume of the seeds (~1/20-1/50 vol) after the polymerization mix has been at 37¡C for 1'. Axonemes or centrosomes can also be used as nucleating structures. If the purpose is labeling or recycling the tubulin, polymerization
on ice. Use the thawed tubulin for polymerization preferably within 30' of thawing. When quantitative analysis of tubulin polymerization is necessary, careful and consistent handling of tubulin aliquots is essential for reproducible results.
the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold room, discard the supernatant. Add 1ml of column buffer containing 0.5 mM GTP to each tube
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