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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
9-Chlorophenanthrene
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
947-72-8
- 规格:
100mg
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
is generally less than 100℃ - which is why PCR works with denaturing temperatures of 91-97℃.A more detailed treatment of annealing / hybridisation is given in an accompanying page, together with explanations of calculations of complexity, conditions
.75 10.92 9.78 9.15 8.75 8.47 8.26 8.10 7.72 7.31 6.88 7 5.59 4.74 4.35 4.12 3.97 3.87 3.79 3.73 3.57 3.41 3.23 12.25 9.55 8.45 7.85 7.46 7.19 6.99 6.84 6.47 6.07 5.65 8 5.32 4.46 4.07 3.84 3.69 3.58 3.50 3.44 3.28 3.12 2.93 11.26 8.65 7.59 7.01
DOP-PCR OF DNA FOR COMPARATIVE GENOMIC HYBRIDIZATION
the tubes, don't take them out; add through the oil) Add 45 ul more oil to cover, flick tubes to make sure there are no bubbles before #95 starts. PCR #95: 1' @ 94 C, 1' @ 56 C, 3' @ 72 C; 35 cycles; link to #96.
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