5-碘-1-戊醇;67133-88-4;98% +(stab

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP

ChIP-Chip E. coli

mg/ml Proteinase K in TBS (store up to 1 year at -20 C) 1 ml 100mM PMSF or 0.5 ml 250 mM Pefabloc   Final concentration For 50ml Lysis Buffer 10mM Tris (pH 8.0) 500μL 1

CHIP PROTOCOL FOR YEAST

currents greater than 90 mA to prevent diffuse bands and heating of the agarose. Reagents Needed for ChIP 37% formaldehyde stock solution 1 M DTT stock 1 M TRIS, pH 9.4 stock 1 mg/mL