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- 文献和实验
- 技术资料
- 保存条件:
RT
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
71607-34-6
- 规格:
100mg
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文献和实验2-D Polyacrylamide Gel Electrophoresis
Thiourea 2 g Chaps 0.5 g Mega 10 0.5 g OBG 250 µl Triton X-100 0.25 g Tris 0.4 g DTT 500 µl Pharmalytes or IPG buffer pH 3-10 (Amersham) 500 µl - mercaptoethanol 4.Add 10 µl tributylphosphine 2 mM,under the hood (2 mM final conc.) 5.Add
Internal standards are extremely valuable for determining the pH and mol-wt ranges of a specific 2-D gel system and for constructing calibration curves for calculating the relative pI and M r of unknown proteins. Although external markers
Computer Analysis of 2-D Images
Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) images, when used to analyze protein expression in large sets of biological samples, are best processed by computer. In this manner, one can detect and quantify even the faintest
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