• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Internal Standards for 2-D

        互联网

        1211
        Internal standards are extremely valuable for determining the pH and mol-wt ranges of a specific 2-D gel system and for constructing calibration curves for calculating the relative pI and M r of unknown proteins. Although external markers are often used for initially calibrating a 2-D gel, eventually the predicted isoelectric points and molecular weights of identified proteins function as calibration markers. For the beginner, several commercial kits are available containing preformulated mixtures of proteins that can be run on 2-D gels either in parallel or mixed with experimental samples as standards. For carrier ampholyte IEF gels, the pH profile can be directly determined by measuring the pH throughout the gel using a standard pH meter. For both CA and IPG IEF gels, a train of differentially carbamylated proteins producing a uniform series of spots on the 2-D gel provides an internal standard for both evaluating the performance of the isoelectric focusing gel and comparing the 2-D spots positions on different gels. However, the exact pI of each spot in the “carbamylated train” is unknown. For calibrating the SDS-PAGE gel, a mixture of protein standards of known molecular weight is simply loaded onto the SDS-PAGE gel flanking both sides of the IEF gel. However, it is important to load the mol-wt standards in the same manner as the IEF gel to minimize any differences in the relative mobilities of the standards and experimental proteins.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序