软骨藻酸;14277-97-5;100ug/ml in ac

v468 Chapter 2 Wnt活性蛋白分离及应用

a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX

V294.PART II，Chapter 2 Boyden法体外趋化试验

, S. V. (1962) The chemotactic effect of mixtures of antibody and antigenon polymorphonuclear leucocytes. J. Exp. Med. 115, 453–466. 2. Carter, S. B. (1967) Heptotaxis and the mechanism of cell motility. Nature 213,256–260. 3. Reiske, H. R

v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性

with 100–200 mL of ultrapure water. 6. Replace water with CBB staining solution, and gently rock thetray (either manually or with a platform rocker). Maximal stainingintensity usually is achieved within 15–30 min, althoughthe gel can be stained overnight