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- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存
- 英文名:
Uniform Silica Microspheres
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
7631-86-9
- 规格:
5ml
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
,NJ), loaded with Cu 2+ . 3. Binding Buffer: 0.5 M NaCl, 20 mM Tris-HCl, pH 7.5, and1% (w/v) CHAPS. 4. Elution Buffer: Binding Buffer with 100 mM imidazole, pH 7.5. 5. Syringe (10 mL) with 0.2-μm pore size filter. 2.3.3. Step 3: Gel
RNA Purification Protocol for 1-2 x 107 cultured cells
temperature. 8. Pour of the supernatant and drain the tube briefly on a clean absorbent paper. Add 3 ml of 70% ethanol and invert the tube few times to wash the RNA pellet. 9. Centrifuge at 3,000-5,000 x g for 10 minutes at room temperature
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