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- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
Plifenat solution
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
21757-82-4
- 规格:
1ml
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single colony, grow overnight at 37?C. Using 3 ul of (overnight growth) single colony
Yeast Protein Isolation "Yaffe-Schatz"
1.Grow 25mls yeast cells to 5x10E6. 2.Sequentially spin down cells in a 15 ml polypro tube. 3.Wash cells with 1ml ice cold ddH2O,transferrring to an eppy tube. 4.Recon.cells in 1 ml ice cold ddH2O containing 100μg/ml PMSF. 5.Add 150μl ice cold
Yeast Protein Isolation "Yaffe-Schatz"酵母总蛋白提取
for 10 min.7.Spin 2 min.in microfuge.Wash pellet with 1ml.ice cold acetone,spin 2 min.and aspirate off the supernatant.Dry pellet.8.Resuspend pellet in 100μl sample buffer: 500μl 3X Sample buffer pH6.8 500μl ddH2O 12.5μl 2-ME 25μl 1M TRIS base 100μg PMSF
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