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for one reaction: The primary PCR cycling conditions are as follows: (a) 95°C for 1 min 30 s (b) 10 cycles of ●● 95°C for 5 s ●● 70°C for 3 min 10 s with decreasing 0.5°C each cycle(c) 20 more cycles of ●● 95°C for 5 s ●● 65°C

免疫沉淀-蛋白印迹法

Methods2.1. Material and reagentsDuolbecco’s phosphate buffered saline (PBS), Medium 199 with Earle’s salts and 25mM HEPES, collagenase Type II (from Clostridum histolyticum), antibiotic/antimyotic solutuion (containing 10,000U penicillin, 10,000µg

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RNA molecules with a short poly(U) tail.In Elbashir's and subsequent publications, siRNAs with other 3' terminal dinucleotide overhangs have been shown to effectively induce RNAi. If desired, you may modify this target site selection strategy to design