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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
WAY-606376
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
522639-94-7
- 规格:
5mg
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文献和实验Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
of primerswith nested primers AKVp8712 and P-nest (see Note 17). The amplification parameters in secondary PCR are changed to: (a) 95°C for 1 min (b) 11 cycles of ●● 94°C for 15 s ●● 70°C for 2 min 40 s with decreasing 0.6°C
Single tube confirmation PCR protocol
on each colony to confirm both of the novel recombination junctions . 1. Clonal purification - Pick three colonies from a transformation plate and streak each one out to single colonies on separate G418 containing plates (200mg/l). - Incubate at 30°C
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