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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
6-Methoxy-2-tetralone
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
2472-22-2
- 规格:
250mg
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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
for one reaction: The primary PCR cycling conditions are as follows: (a) 95°C for 1 min 30 s (b) 10 cycles of ●● 95°C for 5 s ●● 70°C for 3 min 10 s with decreasing 0.5°C each cycle(c) 20 more cycles of ●● 95°C for 5 s ●● 65°C
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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