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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
13-Acetyl-9-dihydrobaccatin III
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
142203-65-4
- 规格:
250mg
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
. Add 4 µg of RNase A and incubate for 5 min at room temperature. Add 3 µL of 10% SDS and 5 µL of 20 mg/mL proteinase K. Incubate at 65°C for 2 h and purify the DNA by phenol:chloroform extraction and ethanol precipitation. Analyze on a 2% agarose
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
anddiluted further to 20 mM as working solutions (see Table 1). Store at -20°C. 12. Agarose powder. 13. Ethidium bromide at 10.0 mg/mL (Invitrogen). Table 1 Sequence (5‘→3') of oligonucleotides used in STA-PCR and SplinkBlunt-PCR
, J. , and Messing, J. ( 1 98 5 ) Improved M 13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vec-tors. Gene 33 , 1 0 3 - 11 9. 35. Olins, P. O. , Devine, C. S. , Rangwala, S. H.
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