13-乙酰基-9-羟基巴卡丁III;142203-65-4;

Native Chromatin Preparation and Illumina/Solexa Library Construction

. Add 4 µg of RNase A and incubate for 5 min at room temperature. Add 3 µL of 10% SDS and 5 µL of 20 mg/mL proteinase K. Incubate at 65°C for 2 h and purify the DNA by phenol:chloroform extraction and ethanol precipitation. Analyze on a 2% agarose

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

anddiluted further to 20 mM as working solutions (see Table 1). Store at -20°C. 12. Agarose powder. 13. Ethidium bromide at 10.0 mg/mL (Invitrogen). Table 1 Sequence (5‘→3') of oligonucleotides used in STA-PCR and SplinkBlunt-PCR  

应用末端截切、进化、再延长技术提高酶稳定性的方法

,  J.  ,  and Messing,  J.  ( 1 98 5 )  Improved M 13 phage  cloning vectors  and  host  strains： nucleotide  sequences  of the  M13mpl8  and  pUC19  vec-tors.  Gene  33 ,  1 0 3 - 11 9. 35.  Olins,  P.  O.  ,  Devine,  C.  S.  ,  Rangwala,  S.  H.