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- 详细信息
- 技术资料
- 品系:
人
- 细胞类型:
正常细胞
- 肿瘤类型:
否
- 供应商:
上海博尔森生物科技有限公司
- 库存:
大量
- 英文名:
ADRB3 Knockout Hela Cell Ripa Lysate (Pool)
- 生长状态:
详见操作说明
- 年限:
12个月
- 运输方式:
4℃
- 器官来源:
人
- 是否是肿瘤细胞:
否
- 细胞形态:
人
- 免疫类型:
详见操作说明
- 物种来源:
人
- 相关疾病:
详见操作说明
- 组织来源:
详见操作说明
- 规格:
50μL, 2μg/μl
ADRB3基因敲除Hela细胞RIPA裂解液(Pool)
ADRB3 Knockout Hela Cell Ripa Lysate (Pool)
Catalog Number BES2006K-1-2PLADRB3 Knockout Hela Cell Ripa Lysate (Pool)
Gene Symbol ADRB3
Synonyms beta 3 Adrenergic Receptor; beta3-adrenergic receptor; beta3-AR; adrenergic; beta-3- receptor; ADR B3; ADRB 3; ADRB3R; Adrenergic beta 3 receptor; B3AR.
Swiss P13945
Gene ID 155
gRNA Species Human
Parental Cell line Hela
Knockout Region E1
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Deletion of the selection cassette in exon 1.
Number of Cells 1 vial knockout cell Lysate and 1 vial parental cell Lysate.
Packaging 1 vial knockout cell Lysate and 1 vial parental cell Lysate.
Shipping Conditions 4℃
Storage Store at -20°C for 6 months storage or -80°C for long-term storage.

Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein.
Protocol Lysates are made using Ripa buffer with 1mM Phenylmethylsulfonyl fluoride (PMSF).
Proteins in this buffer are denatured.To use as WB control, SDS-PAGE loading buffer should be added to the lysate and boiled for 5 minutes, then loaded with gel.
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ADRB3 Knockout Hela Cell Ripa Lysate (Pool)
¥1200










