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- 博尔森生物
- BES2070K-1-1PL
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- 2025年11月26日
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- 文献和实验
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
NOTCH1 Knockout MCF7 Cell Ripa Lysate (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
4℃
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
50μL, 2μg/μl
NOTCH1 Knockout MCF7 Cell Ripa Lysate (Pool)
Gene Symbol NOTCH1
Synonyms activated Notch 1; activated Notch-1;Notch homolog 1 translocation associated (Drosophila); NOTCH1; TAN1; Translocation-associated Notch protein TAN-1.
gRNA Species Human
Parental Cell line MCF7
Knockout Region E6
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.
Applications: WB
Packaging 1 vial knockout cell Lysate and 1 vial parental cell Lysate.
Shipping Conditions 4℃
Storage Store at -20°C for 6 months storage or -80°C for long-term storage.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types.
Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
Protocol Lysates are made using Ripa buffer with 1mM Phenylmethylsulfonyl fluoride (PMSF).
Proteins in this buffer are denatured.To use as WB control, SDS-PAGE loading buffer should be added to the lysate and boiled for 5 minutes, then loaded with gel.
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文献和实验of chromatin fragmentation, which need to be empirically set up for each cell type employed. Remove medium and add 2ml PBS 1X/1mM PMSF to each plate and scrape cells at room temperature (RT). Pool together the cells from two plates and centrifuge
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for 10 minutes at 4°C to pellet debris. 7. Combine supernatants from the two 1.5 ml centrifuge tubes in a new15 ml conical tube for immunoprecipitation. 8. Save 50 µl of cell lysate from each sample as WCE DNA. Store at -20°C. NOTE: Add protease
