2-bromo-4-chloro-6-fluorobenzo

In Situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional -Galactosidase Staining

than 5-bromo-4-chloro-3-indolyl-β-d -galactoside (X-gal). S-gal is advantageous where β-gal activity is limited including early stage mouse embryos. As a result of the increased sensitivity as well as the color compatibility of S-gal, we successfully combined

Human Placental Alkaline Phosphatase Stain

off staining solution with water. STOCK SOLUTIONS XPHOS - 100X solution is 10 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, disodium salt (Sigma) in water, store at -20°C. NBT - 100X solution is 50 mg/ml Nitro Blue tetrazolium (Sigma) in 70

Nonradioactive Northern Blotting

with calorimetric detection via chromogenic substrates (e.g., 5-chloro-4-bromo-indolylphosphate) for alkaline phosphatase.