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- 文献和实验
- 技术资料
- 保存条件:
避光,RT,充氩
- 英文名:
Phenmethylammonium Bromide
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
37488-40-7
- 规格:
250mg
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文献和实验mRNA Purification I. Prepare oligo-dT cellulose use 40 mg oligo-dT cellulose / 1 mg total RNA swell oligo dT-cellulose in elution buffer wash oligo dT-cellulose 4 x with elution buffer (30 sec
bottom tubes. Add a few drops chloroform if the lysate is to be stored at 4°. Otherwise, proceed to part II. H. DNA isolation from lysate: A. Add 10AI 5 mg/ml DNase I and 25 fil 10 mg/ml
HIS-tagged protein purification
and wash resin 3x in 50ml UPB6.3 w/5' gentle shaking each time. 7)resuspend in 25ml UPB6.3,pour into column and elute to top with another 25ml.s 8)elute bound proteins 20ml w/ UPB6.3+250mM imidazole. 9)lN2 freeze and store at 70℃ UPB 1 liter [final
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