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- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存;避光;禁止冻结
- 英文名:
Human IgG1-kappa (Biotin), anti-Spike RBD
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
: Indirect immunohistochemistry with avidin-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 2- Briefly blot the slides without letting
Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
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