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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
1MG

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文献和实验Detection of Glycoproteins on Blot
of EDTA (Tetra sodium salt ) in 100 ml of 200 mM sodium acetate buffer and dilute to 200 ml with Dw 10mM sodium meta periodate solution in 100mM sodium acetate/5mM EDTA Biotin Hydrazide: Prepare a stock solution of 1mg/ml
Immunostaining cells on coverslips
in PBS. Incubate at room temperature for 15 min. 5. Remove fix and quench excess aldehyde by incubation in 1mg/ml sodium borohydride prepared just before use in PBS, pH 8. Incubate in this solution for 15 min at RT. 6. Permeabilize cells
Yeast Chromatin Immunoprecipitation (ChIP)
7.5 140 mM NaCl 1% TritonX-100 0.1% Sodium Deoxycholate 1mM EDTA ChIP lysis buffer (High Salt) (filter sterilize, store at 4 degrees C) 50 mM HEPES pH 7.5 500 mM NaCl 1% TritonX-100 0.1% Sodium Deoxycholate 1 mM EDTA ChIP wash
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