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文献和实验were performed in electrocompetent DH10B or HS996 cells harbouring the pSC101BADγβαA[hygro] or the pSC101BADγβαA-I-SceI[amp] plasmids and using standard Red/ET protocols (8). Electrocompetent cells were prepared as described in the general manuals
: Customizable proteins for DNA targeting. Science 333:1843‐1846. Briggs, A.W., Rios, X., Chari, R., Yang, L., Zhang, F., Mali, P., and Church, G.M. 2012. Iterative capped assembly
Getting a Tree Fast: Neighbor Joining, FastME, and Distance‐Based Methods
. 1997. The performance of the NJ method of phylogeny reconstruction. In Mathematical Hierarchies and Biology (B. Mirkin, F.R. McMorris, F.S. Roberts, and A. Rzhetsky, eds.) pp.133‐148. American Mathematical Society, Providence, R
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