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文献和实验Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO
such as Nω -nitro-L -arginine (L -NNA) and its methyl ester, L -NAME (3 ,4 ). Using the protocol described, this technique is sensitive (limit of detection approx 20 pmol/min per g tissue), specific for NOS in conjunction with the use of NOS inhibitors
The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures
(nNOS). Thus, for the purposes of this chapter, the insult used to induce cell death will be various concentrations of NMDA and the compound used to demonstrate neuroprotection will be the nonspecific NOS inhibitor N ω-nitro-L
Characterization of Calcium Channel Binding
., Patthi, S., Simerson, S., Maroufi, A., McCue, A.F., Velicelebi, G., Ellis, S.B., and Harpold, M.M. 1992. Structure and functional expression of an ω–conotoxin‐sensitive human N‐type calcium channel. Science 257:389‐395
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