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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
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见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
10G

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文献和实验as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
ELISA PROTOCOL 【Yale University 】
Materials:1) TS Buffer: 10 mM Tris pH 8.0150 mM NaCl2) TST Buffer: TS Buffer with 0.05% Tween-203) Alkaline Phosphatase Buffer: 50 mM NaCarbonate1 mM MgCl2(Bring to pH 9.8 with 1 N HCl)4) AP Substrate: p
the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance at 405nm. For HRP conjugates: Mix equal parts of the TMB system, place in wells, allow to develop until desired intensity (app. 5 min) and stop reaction
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