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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
25MG

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文献和实验Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
microcentrifuge Eppendorf tubes. Add 1/10 volume (about 28 µl) of 2 M potassium acetate (pH 5.5). Incubate on ice for 15 minutes. This will removed positively-charged polysaccharides, residual proteins, and other salt-insoluble material.Centrifuge tubes at 10,000
Plasmid DNA Isolation, Alkaline Lysis(碱裂解法分离质粒DNA)
. 3.5 ml 100 µl 20 ml 2. Add soln. 2, mix gently on ice for 5 min. 7 ml 200 µl 40 ml 3. Add soln 3, mix by inversion and leave on ice for 30-60 min. 5.25 ml 150 µl 30 ml 4. Centrifuge for 15 min at 12,000 x g 5. Take supernatant and add 2 vol. cold (-20
µl) of 2 M potassium acetate (pH 5.5). Incubate on ice for 15 minutes. This will removed positively-charged polysaccharides, residual proteins, and other salt-insoluble material. Centrifuge tubes at 10,000 rpm (12,000 g
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