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RT
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联硕生物
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250ML

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文献和实验(0.1 M), 1.6 µl 4dNTP mix (250 µM), 200 ng RNA, 2 µl T12 MN primer (10 pmol/µl), with H2 O to 19 µl incubate for 5 min at 65 °C and for 10 min at 37 °C add 1 µl MMLV reverse transcriptase (200 U/µl) incubate for 50 min at 37 °C
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
,in which case, proceed to the next step. 5. Use 0.5 ml of product from the preceding step to template anested 50 ml PfuTurbo?hot start PCR in (see Note 13) 1×reaction buffer including 300 mM dNTPs, 3.75 U PfuTurbo® DNA Polymerase, and 250 nM GSP2 and GSP
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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