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文献和实验from chloroplast envelopemembranes: a subcellular-specific proteomic approach to identify rare intrinsicmembrane proteins. P la n t J. 19, 217— 228. 1 9 . Gomez, S. M., Nishio, J. N., Faull, K. F., and Whitelegge, J. P. (2002) The chloroplast grana
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
9 small, round clusters of cells. (g ) Day 12 ESC/OP9-DL1 hematopoietic cells. (h ) Day 16 ESC/OP9-DL1 hematopoietic and early T-lineage cells. (i ) Day 20 ESC/OP9-DL1 T-lineage cells. 7. Wash the plate with 4 mL of PBS
centrifugation in density gradients ofPercoll. Arch. Biochem. Biophys. 217, 312-323. 5. Leaver, C. J., Hack, E., and Forde, B. G. (1983) Protein synthesis by isolated plantmitochondria. Methods Enzymol. 97,476-484. 6. Day, D. A., Neuburger, M
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