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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
of Nucleosomal Arrays by Gradient Salt Dialysis Support Protocol 1: Purification of Recombinant Core Histones from Bacteria Support Protocol 2: Preparation of Single 5′ End‐Labeled DNA for Mononucleosome Assembly
thymidine 5′‐triphosphate (TTP, 8t O ). The crude sample can be analyzed on a Welchrom C18 (or Ultisil C18) reversed‐phase column (4.6 × 250 mm) measured at 260 nm at a flow of 1.0 mL/min using a linear gradient of 0% to 40% buffer B in 20 min. Buffer A: 20
Modification of the 5′ Terminus of Oligodeoxyribonucleotides for Conjugation with Ligands
. Bonfils, E. and Thuong, N.T. 1991. Solid‐phase synthesis of 5′‐3′‐bifunctional oligodeoxyribonucleotides bearing a masked thiol group at their 3′‐end. Tetrahedron Lett. 35:3053‐3056
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