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- S278459-10MG
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
10MG
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
further, one or more of the following inhibitors can be tried in addition to heat (they are added to the reaction mix): 0.5 mM levamisole (L[-]-2,3,5,6-tetrahydro-6-phenylimidazo{2,1-b}thiazole), 2 mM mercuric chloride, 5 mM L-leucyl-glycyl-glycine, 1 mM EDTA, 1 mM L-
Metabolic labeling Immunoprecipitation
Purpose and backgrounds About nuclide... 35-S Decay: (b-ray,0.167 MeV) Half life: 87.5 days Thick acrylic shield can block most of the emission.Because of the low energy,it is impossible to detect spillage/contamination by usual
Metabolic labeling Immunoprecipitation
cells twice with Labeling Medium. 2. Add 1.5ml/well Labeling Medium 3. Add 20-30µl (~250µCi) of [35 S] methionine/cysteine /well 4. Culture 1h at 37°C (pulse) 5. Add 1.5ml/well Chase Medium 6. Culture o/n (chase) 7. Harvest metabolically
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