Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(bs-0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-0816R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0816R |
| 英文名称 | Lysozyme Rabbit pAb |
| 中文名称 | 溶菌酶抗体 |
| 英文别名 | 1 4 beta n acetylmuramidase c; 14 beta N acetylmuramidase; 14 beta N acetylmuramidase C; EC 3.2.1.17; lysosyme; Lysozyme C; Lysozyme C precursor; Lyz; LZM; Muramidase. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Chicken) |
| 抗体来源 | Rabbit |
| 免疫原 | Full length native Lysozyme |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 17 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells Immunology > Innate Immunity > Macrophage / Inflamm. Tags & Cell Markers > Subcellular Markers > Organelles > Lysosome |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the glycosyl hydrolase 22 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(bs-0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(bs-0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Raw264.7 Cell (Mouse) Lysate at 40 ug
HL-60 Cell (Human)Lysate at 40 ug
Primary: Anti-Lysozyme (bs-0816R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 16 kD
Raw264.7 Cell (Mouse) Lysate at 40 ug
HL-60 Cell (Human)Lysate at 40 ug
Primary: Anti-Lysozyme (bs-0816R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 16 kD
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文献和实验该产品被引用文献
[IF={{ 8.9 }}] {Huanliang Liu. et al. Oil mistparticulate matter exposure induces hyperlipidemia-related inflammation via microbiota/ SCFAs/GPR43 axis inhibition and TLR4/NF-κB activation. ENVIRON POLLUT. 2024 Jan;:123331} {IHC} {Rat}
[IF={{ 8.713 }}] {Peng Yang. et al. Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes. ELIFE. 2022 Oct} {WB} {Shrimp}
[IF={{ 5.1 }}] {Arong Wang. et al. Lactobacillus-derived indole derivatives ameliorate intestinal barrier damage in rat pups with complementary food administration. FOOD FUNCT. 2024 Aug;:} {IHC} {Rat}
[IF={{ 3.757 }}] {Haruna Nakayama. et al. Preparing a pore-size-controlled TEMPO oxidized cellulose nanofiber substrate for enzyme immunoassay applications. PROCESS BIOCHEM. Process Biochem. 2022 Jun;117:174} {ELISA} {Material}
[IF={{ 1.967 }}] {Nagata, Kenichiro. et al. Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes. ANAL SCI. 2022 Nov;:1-8} {WB} {}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
[151,152,153, 387]Bovine lysozyme c2S, 550 mg/L, native[147]Bovine mannose 6-phosphate receptorsS, α-MF, 28 mg purify. protein/L517Bovine opsinS*, 0.3 mg/L, PHO1[148]Bovine pancreatic trypsin inhibitor (aprotinin)S, 930 mg/L, α-MF[149]Bovine tissue-type
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Lysozyme Rabbit pAb(bs-0816R)-50ul/100ul/200ul
￥1180 - 2800
