IL1R1 | CD121a Rabbit pAb(bs-20697R)-50ul/100ul/200ul产品图
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IL1R1 | CD121a Rabbit pAb(bs-2

0697R)-50ul/100ul/200ul
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  • bs-20697R
  • 2026年10月17日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-20697R
    英文名称IL-1R1 Rabbit pAb
    中文名称白介素1受体1抗体
    英文别名Antigen CD121a; CD121 antigen-like family member A; CD121A; CD121a antigen; D2S1473; IL 1; IL 1R 1; IL 1R alpha; IL 1RT1; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; IL1 inhibitor; IL1R; IL1R1; IL1R1_HUMAN; IL1RA; IL1RN; IL1RT1; Interleukin 1 receptor alpha type 1; Interleukin 1 receptor antagonist protein(IRAP); Interleukin 1 receptor type I; Interleukin receptor 1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; Interleukin-1 receptor type I; P80; IL1 Receptor I; IL-1 Receptor I; Interleukin-1 receptor type 1, soluble form; IL-1 sRI.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human (Mouse, Dog, Pig)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human IL-1R1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量63 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Angiogenesis > Endothelial Cell Markers

    Cardiovascular > Atherosclerosis > Vascular Inflammation > Inflammatory mediators

    Immunology > Innate Immunity > Cytokines > Interleukins

    Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage

    Stem Cells > Hematopoietic Progenitors > Myeloid > Dendritic Cell Lineage

    亚基The interleukin-1 receptor complex is a heterodimer of IL1R1 and IL1RAP.
    亚细胞定位Membrane; Single-pass type I membrane protein. Cell membrane (Probable). Secreted.
    翻译后修饰A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding).
    相似性Belongs to the interleukin-1 receptor family.
    _x000D_ Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    _x000D_ Contains 1 TIR domain.
    功能Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1μg /test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1R1) Polyclonal Antibody, Unconjugated (bs-20697R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    HepG2 cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IL-1R1) polyclonal Antibody, Unconjugated (bs-20697R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片3
    Sample: Hela(human) Cell Lysate at 40 ug
    _x000D_ Primary: Anti-IL-1R1 (bs-20697R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 63 kD
    _x000D_ Observed band size: 63 kD
    _x000D_
    产品细节图片4
    Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
    _x000D_ Primary Antibody (green line): Rabbit Anti-IL-1R1 receptor antibody (bs-20697R) ,Dilution: 0.2μg /10^6 cells;
    _x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
    _x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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    IL1R1 | CD121a Rabbit pAb(bs-20697R)-50ul/100ul/200ul
    ￥1180 - 2800