Blank control:B16.
Primary Antibody (green line): Rabbit Anti-SOX10 antibody (bs-20563R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-20563R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20563R |
| 英文名称 | SOX10 Rabbit pAb |
| 中文名称 | 转录因子SOX10抗体 |
| 英文别名 | DOM; MGC15649; SOX 10; SOX10; SOX10_HUMAN; SRY(sex determining region Y) box 10; SRY box containing gene 10; SRY related HMG box gene 10; Transcription factor SOX 10; Transcription factor SOX-10; WS4. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SOX10 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Lineage specification > Ectoderm Epigenetics and Nuclear Signaling > Transcription > Domain Families > HMG Box Neuroscience > Cell Type Marker > Neural Stem Cell marker Stem Cells > Lineage Markers > Ectoderm |
| 亚细胞定位 | Cytoplasm. Nucleus |
| 组织特异性 | Expressed in fetal brain and in adult brain, heart, small intestine and colon. |
| 相似性 | Contains 1 HMG box DNA-binding domain. |
| 功能 | Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
25 ug total protein per lane of various lysates (see on figure) probed with SOX10 polyclonal antibody, unconjugated (bs-20563R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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文献和实验该产品被引用文献
[IF={{ 4.5 }}] {Bo Li. et al. IF-7 and GALA modified PEG-PDMAEMA loaded SOX10 siRNA nanoparticles: Preparation, cellular delivery, and inhibition on progression of triple negative breast cancer and its bone metastasis in vitro. J DRUG DELIV SCI TEC. 2024 Oct;100:106115} {WB} {Human}
[IF={{ 4.101 }}] {Yun Wang. et al. Prucalopride might improve intestinal motility by promoting the regeneration of the enteric nervous system in diabetic rats. INT J MOL MED. 2022 Jul;50(1):1-10} {IHC} {Rat}
[IF={{ 3.4 }}] {Hongjuan Wang. et al. Differentiation of cultured hair follicle neural crest stem cells into functional melanocytes. HELIYON. 2024 Aug;10:} {IF} {Mouse}
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SOX10 Rabbit pAb(bs-20563R)-50ul/100ul/200ul
￥1180 - 2800
