- ￥99 - 999
- Sigma
- 进口
- S7809-5MG
- 2025年10月24日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
5MG
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
) through the oil to sample. (It's 0.3 ul per 15ul original volume, so adjust as necessary). Incubate overnight at 55 C. Day 3: (Repeat:) Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock) through the oil to sample. (It's 0.3 ul per 15 ul original
Yale RNA prep (Rebecca D. Burdine, Michael J. Stern [modified by S.M. Hettenbach]) Add 8 ml of TRIZOL to 2 ml packed worms in 15 ml centrifuge tube. Vortex and invert tube to solubilize and lyse worms for at least 10 min.
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
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