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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
鹿森生物
- 规格:
10MG
Application
Fumonisin B2 (FB2) from Fusarium moniliforme has been used:
as a standard for the detection of FB2 by high performance liquid chromatography (HPLC) in corn based food products[1][2]
used as standard in the determination of fumonisin B1 and B2 in corn and corn-based products by high-performance liquid chromatography[2]
as a standard in the determination of mycotoxins by HPLC[3]
Biochem/physiol Actions
Inhibitor of sphingosine N-acyltransferase. Blocks the formation of ceramide from sphingosine.
General description
Fumonisin B2 (FB2) is a diester of tricarballylic acid and polyhydric alcohols.[1]. Fumonisins B2 (FB2) does not have one or more hydroxyl groups at the C5 or C10 positions.[4]
Mycotoxin originally isolated from Fusarium sp, which grows on corn. Produces neural tube defects and craniofacial abnormalities in fetuses. Many strains of Aspergillus niger growing naturally on grapes are capable of producing fumonisin B2, so it is now a concern for human consumption of grapes, raisins, and wine.
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文献和实验的表面涂上一层右旋糖酐或蛋白类化合物。这样活性炭末的表面构成一定大小的孔洞,在反应液中加入这种特殊颗粒的混悬液时,小分子的游离抗原被活性炭吸附,达到分离B与F的目的。 活性炭吸附方法的优点是操作简便、价廉,缺点是离心沉淀部分是F而不是B,不适用目前自动化放免测定,分离时易受温度和时间的影响。 (二)沉淀分离法 沉淀分离法的原理,是盐类或有机溶剂能够破坏蛋白质分子表面的水化层而发生沉淀(这种分离要求蛋白质分子处在等电点环境中)。常用的沉淀剂是聚乙二醇(PEG
Purification of Myosin Light Chain Kinase
inhibitor* - 8ml of 1mg/ml stock solution pH 7.0 3.Buffer B DE 52 Wash 10 mM MOPS- 20ml of 2M stock solution 4 liters 1 mM EDTA- 20ml of 0.2M stock solution 1 mM DTT* - 4ml of 1M stock solution 0.1 mM PMSF* - 7ml of 10mg/ml stock solution pH
/mL。 f、基于以下两个原因,建议准备2倍于正常共孵育实验所需外泌体的量来进行染料标记。一是使用过量染料进行标记,外泌体的染色效率仍无法达到 100%;二是去除游离染料的过程中存在外泌体回收率的损失。 21、关于外泌体荧光染料(DIR)相关实验注意事项 a、活体成像对外泌体浓度要求较高,建议用于染料标记的外泌体原始浓度达到 0.5~1.5μg/μL,外泌体浓度过低实验失败风险较高。 b、外泌体染色步骤中去除游离染料的第 4、5 步必不可少,以避免游离染料对后期实验的干扰。这一步相当于重新提取外泌
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