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文献和实验Secondary structure of prion mRNA
with canonical tRNA. Nucl. Acids Res. 10, 19291946. Schaƴzl, H. M., Da Costa, M., Taylor, L., Cohen, F. E. & Prusiner, S. B. (1995). Prion protein gene variation among primates. J. Mol. Biol. 245, 362374. Schmitz, M. & Steger, G. (1992). Base-pair probability
Fast and reliable mini-prep RNA extraction from Neurospora crassa
containing high levels of nucleases. Extraction from starved cultures or plate mycelia with the LiCl method (Chambers and Russo, Fungal Genet. Newsl. 1987. 33:22-24) gave completely degraded RNA as judged by gel electrophoresis. In trying to improve
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
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