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- 2025年10月23日
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- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
100ML
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文献和实验. MMS has a short aqeuous half-life Henderson Method -- approx. 1-5% integrants Make a solution of 0.1% (1 l in 1 ml) MMS in M9. Put 100 l of 0.1% MMS in a thumb depression plate. Pick 35-40 L4 non-Uncs
in late L4. Worms need to be staged as precisely as possible, because this seems to reduce fluctuation. 2. Incubate worms at 20 ºC for 30 hrs. 3. Wash worms off plates with 2.5 ml M9 buffer at a time x 2 times, combine the washes in a 15 ml falcon
in late L4. Worms need to be staged as precisely as possible, because this seems to reduce fluctuation. 2. Incubate worms at 20 ºC for 30 hrs. 3. Wash worms off plates with 2.5 ml M9 buffer at a time x 2 times, combine the washes in a 15 ml falcon
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