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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
250MG

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文献和实验221.3 10.4 9.7-11.1 * pKa=9.0 for the second stage of dissociation. ATP C10 H14 N5 O13 P3 Na2 Mw=551.1 g/M; (store at -20o C). Conc. Stock 1ml ATP 0.1M 551.1g/M 55.1mg H2 O mQ
Home-made Taq Polymerase Purification
( 8K / 15mins / 4°C). 7) Add 1.86mg of KCl / ml of supernatant. 8) Aliquot equal volumes of supernatant into each of 2 x 250ml centrifuge tubes (preferably conical) containing 75ml of washed Sigma DP-1 cation exchange resin (packed
2 O to 1 L 1 M DTT (Dithiothreitol, Cleland's reagent): 1.54 g DTT (Calbiochem 233155) ddH2 O to 10 ml (aliquot and store at -20℃). DNase-free RNase A 20 mg/ml RNase A in 1 mM NaOAc, pH 4.5. 200 mg RNase A (Sigma R-5500) 3.3 μl 3 M NaOAc, pH
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