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- 2025年10月23日
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联硕生物
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文献和实验Protein Gel Preparation and Staining
-379) is preferable. Solutions 30% Acrylamide 29 g acrylamide 1 g Bis acrylamide up to 100 ml with Q store at room temperature in a light proof bottle 4X Resolving Gel Buffer 1.5 M Tris pH 8.8 18.17 g
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
method (6) and the other gel slice was transferred into microcentrifuge tube and then completely crushed by a disposable pipette tip. In the next step, 100-200 µl volume of phenol/chloroform/isoamyl alcohol (25:54:1) was added to the microcentrifuge tube
SSR GEL and Silver Staining Protocol
. 2. Gels are 6% acrylamide, 8M urea, 1X TBE. For each gel, mix together 50 g urea, 15 ml 40% 19:1 acrylamide solution, and 31 ml d.i. water. We use a 4.5% gel for fingerprinting reactions. Note: (We store aliquots of premixed 40% Acrylogel solution
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