- 询价
- BIO-RAD
- MCA835GA
- 2025年10月17日
- C, F, IP
- Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Bovine thymocytes
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Bovine
- 保质期：
自发货之日起 12 个月
- 目录编号：
CC17
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, F, IP
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD5 antibody | CC17
- 规格：
0.1 mg
Mouse anti Bovine CD5 antibody, clone CC15 recognizes bovine CD5, a ~67 kDa type 1 single pass transmembrane molecule containing three scavenger receptor cysteine rich (SRCR) domains. Clone CC17 reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos taurus animals and a small proportion of Bos indicus animals.
Bo5.1, recognized by Mouse anti Bovine CD5, clone CC17 is the only isoform expressed by Bos taurus while Bos indicus may express Bo5.2 or both allelic forms of bovine CD5. (Howard et al.1989) .
Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes (Howard et al. 1988).
Bo5.1, recognized by Mouse anti Bovine CD5, clone CC17 is the only isoform expressed by Bos taurus while Bos indicus may express Bo5.2 or both allelic forms of bovine CD5. (Howard et al.1989) .
Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes (Howard et al. 1988).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/25 | 1/100 |
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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文献和实验该产品被引用文献
References for CD5 antibody
- Howard, C.J. et al. (1988) Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5.
Vet Immunol Immunopathol. 19(2): 127-39. - Howard, C.J. & Leibold, W. (1991) Individual antigens of cattle. Bovine CD5 (BoCD5).
Vet Immunol Immunopathol. 27(1-3): 55-60. - Howard, C.J. et al. (1991) Competitive binding with putative Bo5 (CD5) cluster of monoclonal antibodies.
Vet Immunol Immunopathol. 27(1-3): 147-51. - Hein, W.R. et al. (1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species.
Vet Immunol Immunopathol. 27(1-3): 32-4. - Chevallier, N. et al. (1998) Bovine leukemia virus-induced lymphocytosis and increased cell survival mainly involve the CD11b+ B-lymphocyte subset in sheep.
J Virol. 72(5): 4413-20. - Haas, K.M. & Estes, D.M. (2000) Activation of bovine B cells via surface immunoglobulin M cross-linking or CD40 ligation results in different B-cell phenotypes.
Immunology 99: 272-8. - Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41(1): 4. - Nfon, C.K. et al. (2012) Innate immune response to Rift Valley fever virus in goats.
PLoS Negl Trop Dis. 6: e1623.
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文献支持
Mouse anti Bovine CD5，clone CC17
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