- 询价
- BIO-RAD
- MCA5941GA
- 2025年10月17日
- C, E, IF, WB
- Sheep
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant ovine IgE1-2 expressed in E.coli
- 亚型：
IgG2a
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Sheep
- 保质期：
自发货之日起 12 个月
- 目录编号：
1E7
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, E, IF, WB
- 浓度：
IgG concentration 0.5 mg/ml
- 抗体名：
IgE antibody | 1E7
- 规格：
0.1 mg
Mouse anti Sheep IgE, clone 1E7, recognizes ovine Immunoglobulin E (IgE) and does not cross react with ovine IgM, IgA, IgG1 or IgG2.
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman et al. 1997).
IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep by Haemonchus contortus (Vervelde et al. 1997 and Kooyman et al. 1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello et al. 2007 and Kooyman et al. 2002).
In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman et al. 1997).
IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep by Haemonchus contortus (Vervelde et al. 1997 and Kooyman et al. 1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello et al. 2007 and Kooyman et al. 2002).
In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 1/500 | 1/2000 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Western Blotting | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
References for IgE antibody
- Kooyman, F.N. et al. (1997) Production of a monoclonal antibody specifc for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection.
Parasitology 114:395-406 - Kooyman, F.N. et al. (2002) Serum immunoglobulin E response in calves infected with the lungworm Dictyocaulus viviparus and its correlation with protection.
Parasite Immunol. 24:47-56 - Vervelde, L. et al. (2003) Vaccination-induced protection of lambs against the parasitic nematode Haemonchus contortus correlates with high IgG antibody responses to the LDNF glycan antigen.
Glycobiology 13:795-804 - Bricarello, P.A. et al. (2007) Field study on nematode resistance in Nelore-breed cattle.
Vet Parasitol.148:272-8
技术资料暂无技术资料 索取技术资料
文献支持
Mouse anti Sheep IgE，clone 1E7
询价
