- 询价
- BIO-RAD
- MCA1825F
- 2025年10月17日
- F
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
T-end.1, a pMT transformed endothelial cell line.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
MEC14.7
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD34 antibody | MEC14.7
- 规格：
0.1 mg
Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
-
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
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文献和实验该产品被引用文献
Source Reference
- Garlanda, C. et al. (1997) Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
Eur J Cell Biol. 73 (4): 368-77.
References for CD34 antibody
- Winding, B. et al. (2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.
Clin Cancer Res. 8 (6): 1932-9. - Nguyen, L. et al. (2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.
BMC Cancer. 12: 522. - Morison, N.B. et al. (2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.
Reproduction. 133: 309-21. - Chen, L. et al. (2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.
Am J Pathol. 177: 2671-80. - Ager, E.I. et al. (2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.
Cancer Cell Int. 10: 19 - Chabot, S. et al. (2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.
J Exp Med. 208: 973-86. - Chen, J. et al. (2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.
Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71. - Chen, J. et al. (2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.
PLoS One. 7 (11): e50105.
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文献支持
Rat anti Mouse CD34:FITC，clone MEC14.7
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