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- MCA1019SBB615
- 2025年12月19日
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Namalwa human B-cell line.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to StarBright Blue 615 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright Blue 615
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
AT13/5
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD38 antibody | AT13/5
- 规格：
100 Tests/0.5ml
小鼠抗人CD38抗体（克隆AT13/5）可识别CD38分子。该分子亦称ADP-核糖基环化酶/cADP核糖水解酶1、2'-磷酸-ADP核糖基环化酶、ADP-核糖基环化酶1、环ADP核糖水解酶1或T10蛋白。CD38是由300个氨基酸组成的约45 kDa单次跨膜II型糖蛋白，且本抗体的反应模式与CD38抗体簇中其他抗体一致。该蛋白在浆细胞、单核细胞、早期淋巴细胞及活化T细胞中均有表达。
CD38分子被广泛用于研究B细胞与T细胞的分化及活化过程。作为一种多功能酶，CD38参与钙离子信号传导及烟酰胺腺嘌呤二核苷酸（NAD+）代谢过程（de Oliveira et al. 2018）。
| 项目 | 内容 |
|---|---|
| 靶标物种 | 人 |
| 产品形式 | StarBright Blue 615 标记的纯化IgG - 液体 |
| 制备方法 | 通过Protein A亲和层析从组织培养上清中纯化IgG |
| 缓冲溶液 | 磷酸盐缓冲盐水 |
| 防腐剂与稳定剂 | 0.09% (NaN₃)；1%牛血清白蛋白；0.1% Pluronic F68；0.1% PEG 3350；0.05% Tween 20 |
| 免疫原 | Namalwa人B细胞系 |
| 近似蛋白浓度 | 关于StarBright染料偶联试剂的浓度信息，请访问我们的FAQ页面。 |
| 融合细胞系 | 免疫的BALB/c小鼠脾细胞与小鼠NS1骨髓瘤细胞融合 |
| 最大激发/发射波长 | 荧光染料：StarBright Blue 615 最大激发波长 (nm)：475 最大发射波长 (nm)：612 |
| 监管声明 | 仅供研究使用 |
| 保质期 | 自发货之日起12个月 |
| 专利声明 | 本产品受美国专利号10,150,841及相关美国和外国同等专利的保护 |
储存信息
本品以环境温度运输。储存于+4°C环境，切勿冷冻。本产品应原液保存。
应用
经实验室检测、同行评审文献或研发者提供的信息确认，本产品在以下应用中表现良好。详细信息可查阅参考文献，常规实验方案建议请参见抗体使用指南页面。
| 应用名称 | 已验证 | 建议最低稀释度 | 建议最高稀释度 |
|---|---|---|---|
| 流式细胞术 | ✓ | 原液使用 |
若产品未针对特定技术进行测试，并不排除其在该技术中使用的可能性。建议工作稀释度仅供参考，推荐用户结合自身实验体系通过阴性/阳性对照进行滴度优化。
流式细胞术应用建议：
取推荐工作稀释度的抗体5μl，加入100μl细胞悬液（约含10⁶个细胞）进行标记。建议最佳实践是在上样前以6,000g离心5分钟处理样本。
Mouse anti Human CD38 antibody, clone AT13/5 recognizes CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1 or T10. CD38 is a 300 amino acid ~45 kDa single pass type II transmembrane glycoprotein and Mouse anti Human CD38 antibody, clone AT13/5 has a reactivity pattern consistent with other antibodies of the CD38 cluster. CD38 is expressed by plasma cells, monocytes, early lymphoid cells and activated T cells.
CD38 is widely used to study the processes of B and T differentiation and activation. CD38 is a multifunctional enzyme involved in calcium signaling and nicotinamide adenine dinucleotide (NAD+) metabolism (de Oliveira et al. 2018).
CD38 is widely used to study the processes of B and T differentiation and activation. CD38 is a multifunctional enzyme involved in calcium signaling and nicotinamide adenine dinucleotide (NAD+) metabolism (de Oliveira et al. 2018).
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
Source Reference
- Ellis, J.H. et al. (1995) Engineered anti-CD38 monoclonal antibodies for immunotherapy of multiple myeloma.
J Immunol. 155 (2): 925-37.
References for CD38 antibody
- Corcione, A. et al. (2009) CX3CR1 is expressed by human B lymphocytes and mediates [corrected] CX3CL1 driven chemotaxis of tonsil centrocytes.
PLoS One. 4: e8485. - Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12: 2. - Tipping, A.J. et al. (2009) High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle
Blood. 113: 2661-72. - Slyker, J.A. et al. (2012) Acute cytomegalovirus infection is associated with increased frequencies of activated and apoptosis-vulnerable T cells in HIV-1-infected infants.
J Virol. 86 (20): 11373-9. - Slyker, J.A. et al. (2012) The impact of HIV-1 infection and exposure on natural killer (NK) cell phenotype in Kenyan infants during the first year of life.
Front Immunol. 3: 399. - Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
J Cancer Therapy. 02 (01): 54-64.
Further Reading
- Malavasi, F. et al. (1994) Human CD38: a glycoprotein in search of a function.
Immunol Today. 15 (3): 95-7.
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小鼠抗人CD38抗体：StarBright Blue 615荧光标记
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