Human anti Chicken CD25:FITC,clone AbD13504
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Human anti Chicken CD25:FITC,c

lone AbD13504
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  • 询价
  • BIO-RAD已认证
  • HCA173F
  • 2025年10月17日
  • F
  • Chicken
  • 详细信息
  • 文献和实验
  • 技术资料
  • 免疫原

    Recombinantly expressed his-tagged chicken CD25

  • 亚型

    HuCAL Fab bivalent

  • 形态

    A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.

  • 克隆性

    Monoclonal Antibody

  • 标记物

    FITC

  • 适应物种

    Chicken

  • 保质期

    自发货之日起 12 个月

  • 目录编号

    AbD13504

  • 供应商

    伯乐生命医学产品(上海)有限公司

  • 应用范围

    F

  • 浓度

    Antibody concentration 0.1 mg/ml

  • 抗体名

    CD25 antibody | AbD13504

  • 规格

    0.1 mg

Human anti Chicken CD25 antibody, clone AbD13504 recognizes the chicken IL-2 receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.


储存信息

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.


应用

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.


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图标文献和实验
该产品被引用文献

References for CD25 antibody

  1. Braukmann, M. et al. (2015) Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
    Vet Immunol Immunopathol. 168 (1-2): 14-8.
  2. Polasky C et al. (2016) Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
    Vet Immunol Immunopathol. 179: 1-7.
  3. Lee, I.K. et al. (2018) Regulation of CD4+CD8-CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken.
    Sci Rep. 8 (1): 8627.
  4. He, S. et al. (2021) High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infection.
    Vet Res. 52 (1): 119.
  5. Rimondi, A. et al. (2022) Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry.
    Front Microbiol. 13: 953738.
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Human anti Chicken CD25:FITC,clone AbD13504
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