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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinantly expressed his-tagged chicken CD25
- 亚型:
HuCAL Fab bivalent
- 形态:
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Chicken
- 保质期:
自发货之日起 12 个月
- 目录编号:
AbD13504
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
Antibody concentration 0.1 mg/ml
- 抗体名:
CD25 antibody | AbD13504
- 规格:
0.1 mg
Human anti Chicken CD25 antibody, clone AbD13504 recognizes the chicken IL-2 receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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文献和实验该产品被引用文献
References for CD25 antibody
- Braukmann, M. et al. (2015) Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
Vet Immunol Immunopathol. 168 (1-2): 14-8. - Polasky C et al. (2016) Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
Vet Immunol Immunopathol. 179: 1-7. - Lee, I.K. et al. (2018) Regulation of CD4+CD8-CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken.
Sci Rep. 8 (1): 8627. - He, S. et al. (2021) High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infection.
Vet Res. 52 (1): 119. - Rimondi, A. et al. (2022) Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry.
Front Microbiol. 13: 953738.
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文献支持
Human anti Chicken CD25:FITC,clone AbD13504
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