- 询价
- BIO-RAD
- MCA2316F
- 2025年10月17日
- F
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine alveolar macrophages.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
3B11/11
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD169 antibody | 3B11/11
- 规格：
0.1 mg
Mouse anti Pig CD169, clone 3B11/11 recognizes porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003) .
Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.
Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011) .
Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009).
Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.
Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011) .
Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
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文献和实验该产品被引用文献
Source Reference
- Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13.
References for CD169 antibody
- Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. - Perdiguero, B. & Blasco, R. (2006) Interaction between vaccinia virus extracellular virus envelope A33 and B5 glycoproteins.
J Virol. 80 (17): 8763-77. - Perdiguero, B. et al. (2008) Vaccinia virus A34 glycoprotein determines the protein composition of the extracellular virus envelope.
J Virol. 82 (5): 2150-60. - Revilla, C. et al. (2009) Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 40 (3): 14. - Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
Dev Comp Immunol. 33 (3): 284-98. - Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46. - Costa-Hurtado, M. et al. (2013) Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence.
Infect Immun. 81 (7): 2327-33. - Rodríguez-Gómez IM et al. (2015) PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate.
Vet Res. 46: 54.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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Mouse anti Pig CD169:FITC，clone 3B11/11
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