- 询价
- BIO-RAD
- MCA2600F
- 2025年10月17日
- F
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine bone marrow hematopoietic cells (BMHC)
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
2B2
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1mg/ml
- 抗体名：
Granulocytes antibody | 2B2
- 规格：
0.1 mg
Mouse anti Pig granulocytes antibody, clone 2B2 recognizes porcine basophils, neutrophils and eosinophils, acting as a reliable tool for their analysis and isolation, without contamination from other cells.
Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code (MCA2599GA), allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins (Pérez et al. 2007).
Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code (MCA2599GA), allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins (Pérez et al. 2007).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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文献和实验该产品被引用文献
References for Granulocytes antibody
- Nguyen, D.N. et al. (2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
Sci Rep. 6: 36816. - LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22. - Andersen, A.D. et al. (2019) Synbiotics Combined with Glutamine Stimulate Brain Development and the Immune System in Preterm Pigs.
J Nutr. 149 (1): 36-45.
Further Reading
- Pérez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
Dev Comp Immunol. 31 (3): 296-306. - Piriou-guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39 (6): 54.
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文献支持
Mouse anti Pig Granulocytes:FITC，clone 2B2
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