- 询价
- BIO-RAD
- MCA773F
- 2025年10月17日
- F
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rat Ax cells (a HEV derived cell line).
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Rat
- 保质期：
自发货之日起 12 个月
- 目录编号：
1A29
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD54 antibody | 1A29
- 规格：
0.1 mg
Mouse anti Rat CD54 antibody, clone 1A29 recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a ~90 kDa adhesion molecule belonging to the immunoglobulin superfamily.
CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and plays an important role in various cell-cell interactions in the immune system. Cross-linking of ICAM-1 using clone 1A29 induces calcium signaling (Etienne et al. 1998).
Mouse anti Rat CD54 antibody, clone 1A29 inhibits homotypic aggregation of cells including PHA blasts (Tamatani & Miyasaka 1990).
CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and plays an important role in various cell-cell interactions in the immune system. Cross-linking of ICAM-1 using clone 1A29 induces calcium signaling (Etienne et al. 1998).
Mouse anti Rat CD54 antibody, clone 1A29 inhibits homotypic aggregation of cells including PHA blasts (Tamatani & Miyasaka 1990).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
References for CD54 antibody
- Etienne, S. et al. (1998) ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells.
J Immunol. 161 (10): 5755-61. - Kawai, T. et al. (1999) Selective diapedesis of Th1 cells induced by endothelial cell RANTES.
J Immunol. 163: 3269-78. - Adamson, P. et al. (1999) Lymphocyte migration through brain endothelial cell monolayers involves signaling through endothelial ICAM-1 via a rho-dependent pathway.
J Immunol. 162: 2964-73. - Sato, N. et al. (2000) Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury.
Am J Respir Crit Care Med. 161: 1681-8. - Etienne-Manneville, S. et al. (2000) ICAM-1-coupled cytoskeletal rearrangements and transendothelial lymphocyte migration involve intracellular calcium signaling in brain endothelial cell lines.
J Immunol. 165 (6): 3375-83. - Arsenović-Ranin, N. et al. (2000) A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes.
Immunology. 100: 334-44. - Beck-Schimmer, B. et al. (2001) Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells.
Am J Respir Cell Mol Biol. 25: 780-7. - McKechnie, N. M. et al. (2002) Antigenic mimicry: Onchocerca volvulus antigen-specific T cells and ocular inflammation.
Invest Ophthalmol Vis Sci. 43:411-8.
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文献支持
Mouse anti Rat CD54:FITC，clone 1A29
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