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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human E-Selectin (ELAM-1).
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
1.2B6
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD62E/CD62P antibody | 1.2B6
- 规格：
25 µg/0.1 mg
| 规格： | 25 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.1 mg | 产品价格： | 询价 |
Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens.
Although initially thought to recognize only human CD62E, more recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.
Clone 1.2B6 reacts with porcine E-selectin (CD62E) but not with porcine P-selectin (Stocker et al. 2000).
Although initially thought to recognize only human CD62E, more recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.
Clone 1.2B6 reacts with porcine E-selectin (CD62E) but not with porcine P-selectin (Stocker et al. 2000).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
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文献和实验该产品被引用文献
Source Reference
- Wellicome, S.M. et al. (1990) A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide.
J Immunol. 144 (7): 2558-65.
References for CD62E/CD62P antibody
- Thornhill, M.H. & Haskard, D.O. (1990) IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma.
J Immunol. 145 (3): 865-72. - Keelan, E.T. et al. (1994) Characterization of E-selectin expression in vivo with use of a radiolabeled monoclonal antibody.
Am J Physiol. 266 (1 Pt 2): H278-90. - Kyan-Aung, U. et al. (1991) Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo.
J Immunol. 146 (2): 521-8. - Gómez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis.
J Virol. 73: 2173-80. - Goda, K. et al. (1999) Characterization of an apparently conserved epitope in E- and P-selectin identified by dual-specific monoclonal antibodies.
Eur J Immunol. 29 (5): 1551-60. - Stocker, C.J. et al. (2000) TNF-alpha, IL-4, and IFN-gamma regulate differential expression of P- and E-selectin expression by porcine aortic endothelial cells.
J Immunol. 164: 3309-15. - Vallée, I. et al. (2001) African swine fever virus infection of porcine aortic endothelial cells leads to inhibition of inflammatory responses, activation of the thrombotic state, and apoptosis.
J Virol. 75: 10372-82. - Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther 321: 656-62.
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Mouse anti Human CD62E/CD62P:FITC，clone 1.2B6
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