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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fc-fusion protein containing the sequence 21-588 from bovine TLR2
- 亚型:
HuCAL Fab bivalent
- 形态:
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to fluorescein isothiocyanate (FITC) - liquid.
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Bovine
- 保质期:
自发货之日起 12 个月
- 目录编号:
AbD12542
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
Ig concentration 0.1 mg/ml
- 抗体名:
CD282 antibody | AbD12542
- 规格:
0.1 mg
Human anti Bovine CD282 antibody, clone AbD12542 recognizes bovine TLR2, otherwise known as CD282. TLR2 is a single-pass type 1 membrane protein belong to the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
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文献和实验该产品被引用文献
References for CD282 antibody
- Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139: 313-8. - Garza-Cuartero, L. et al. (2016) Fasciola hepatica infection reduces Mycobacterium bovis burden and mycobacterial uptake and suppresses the pro-inflammatory response.
Parasite Immunol. 38 (7): 387-402. - Conejeros, I. et al. (2015) Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.
Dev Comp Immunol. 52 (2): 215-25. - Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 12 (10): 924.
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文献支持
Human anti Bovine CD282:FITC,clone AbD12542
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