- 询价
- BIO-RAD
- MCA1525A488
- 2025年10月17日
- F *
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified CHO cell expressed recombinant human IL-4.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Alexa Fluor® 488 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Alexa Fluor® 488
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
MP4-25D2
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F *
- 浓度：
IgG concentration 0.05 mg/ml
- 抗体名：
IL-4 antibody | MP4-25D2
- 规格：
100 Tests/1ml
Rat anti Human Interleukin-4 antibody, clone MP4-25D2 recognizes human Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, Binetrakin, Pitrakinra or Lymphocyte stimulatory factor 1. Interleukin-4 is a 153 amino acid ~17kDa cytokine involved in the activation of B cells, macrophages and monocytes (
UniProt: P05112).
Polymorphisms in the IL-4 gene are associated with susceptibility to, and outcome of ischemic stroke ( Marousi et al. 2011).
Rat anti Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity ( Abrams et al. 1992 and Carvalho et al. 1994).
Polymorphisms in the IL-4 gene are associated with susceptibility to, and outcome of ischemic stroke ( Marousi et al. 2011).
Rat anti Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity ( Abrams et al. 1992 and Carvalho et al. 1994).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | ✓ | NT | 1/10 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
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文献和实验该产品被引用文献
References for IL-4 antibody
- Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24. - Carvalho, E.M. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
J Immunol. 152 (12): 5949-56. - P, M. et al. (2004) Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
J Immunol. 172 (12): 7341-9. - Raulf-Heimsoth, M. (2008) T cell - primary culture from peripheral blood.
Methods Mol Med. 138: 17-30.
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文献支持
Rat anti Human Interleukin-4:Alexa Fluor® 488，clone MP4-25D2
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