- 询价
- BIO-RAD
- MCA1971SBV570
- 2025年12月11日
- F
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine peripheral blood leucocytes
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to StarBright Violet 570 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
StarBright Violet 570
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
G7
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- 抗体名：
CD16 antibody | G7
- 规格：
100 Tests/0.5ml
Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).
Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
储存信息
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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文献和实验该产品被引用文献
Source Reference
- Dato, M.E. et al. (1992) A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.
Cell Immunol. 140 (2): 468-77.
References for CD16 antibody
- Wierda, W.G. et al. (1993) Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex.
Cell Immunol. 146 (2): 270-83. - Halloran, P.J. et al. (1994) Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7.
Cell Immunol. 158 (2): 400-13. - Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7. - Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
Vet Res Commun. 26: 273 - 83. - Vincent, I.E. et al. (2003) Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus.
J Virol. 77: 13288 - 300. - Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology 110: 440-9. - Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
Clin Exp Immunol. 160 (3): 431-9. - Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88 (3): 571-8.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54. - Gerner W et al. (2015) Phenotypic and functional differentiation of porcine αβ T cells: current knowledge and available tools.
Mol Immunol. 66 (1): 3-13.
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Mouse anti Pig CD16:StarBright Violet 570
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