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- bsm-43117M
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bsm-43117M |
| 英文名称 | CD63 Mouse mAb |
| 中文名称 | 黑色素瘤相关抗原单克隆抗体 |
| 英文别名 | Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63 antigen(melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LAMP-3; LIMP; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; PTLGP40; Tetraspanin-30; Tspan-30; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; MGC72893; MLA 1; MLA1; ocular melanoma associated antigen; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30; Tumor biomarkers; Platelets markers. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human CD63 protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 26 kDa |
| 实际分子量 | 30-50kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Cell adhesion Cancer > Tumor biomarkers > Tumor antigens Cardiovascular > Blood > Platelets Immunology > Cell Type Markers > CD > Platelets Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. |
| 组织特异性 | Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. |
| 相似性 | Belongs to the tetraspanin (TM4SF) family. |
| 功能 | This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
. Use gloves and aerosol barrier tips. Linearize the plasmid and check the digest. Phenol extract. Extract twice with chloroform:isoamyl alcohol (24:1) Ethanol precipitate ( 1/2 vol 7.5 M NH4 OAc + 2.5 vol 100
Hybrid Resistance by Mouse NK Cells In Vitro
A purified NK cells to kill H2d target cells can be reversed by adding MAb reagents that bind to either Ly49A or H2-Dd . We and others have extended these early findings to the Ly49C and I subsets of NK cells, and have recently detected positive signaling
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